Table 9. IPA target gene analysis on miRNAs specifically altered between normal tissue and cancers of BRCA1 carriers and selected for qRT-PCR.
| miRNA | Targeted genes | Associated pathways |
|---|---|---|
| hsa-miR-153 | ACVR1B | Epithelial adherens junction, PPARα/RXRα activation, TGF-β signaling, Wnt/β-catenin signaling |
| BCL2 | Apoptosis, glucocorticoid receptor signaling, p53 signaling, PEDF signaling, PI3K/AKT signaling, PTEN signaling, TGF-β signaling, VEGF signaling | |
| CBX5 | ATM signaling | |
| DNM3 | Clathrin-mediated endocytosis signaling, remodeling of epithelial adherens junctions | |
| DVL3 | Embryonic stem cell pluripotency, regulation of the epithelial-mesenchymal transition pathway, Wnt/β-catenin signaling | |
| FOXO3 | Glucocorticoid receptor signaling, IGF-1 signaling, insulin receptor signaling, PI3K/AKT signaling, PTEN signaling, PXR/RXR activation, VEGF signaling | |
| RAB7A | Clathrin-mediated endocytosis signaling, remodeling of epithelial adherens junctions | |
| SNAI1 | Epithelial adherens junction signaling, ILK signaling, regulation of the epithelial-mesenchymal transition pathway | |
| hsa-miR-183-5p | BTRC | Cyclins and cell cycle regulation, NF-κB signaling, Wnt/β-catenin signaling |
| FGF9 | Actin cytoskeleton signaling, clathrin-mediated endocytosis signaling, FGF signaling, regulation of the epithelial-mesenchymal transition pathway | |
| FOXO1 | ErbB signaling, IGF-1 signaling, PI3K/AKT signaling, PTEN signaling, VEGF signaling | |
| PRKACB | Breast cancer regulation by Stathmin1, CDK5 signaling, NF-κB signaling PPARα/RXRα activation, tight junction signaling, cAMP-mediated signaling, eNOS signaling | |
| RAD50 | ATM signaling, DNA double-strand break repair by homologous recombination, DNA double-strand break repair by non-homologous end joining, hereditary breast cancer signaling, role of BRCA1 in DNA damage response, role of CHK proteins in cell cycle checkpoint control, telomere extension by telomerase | |
| hsa-miR-21-5p | ACTA2 | Actin cytoskeleton signaling, gap junction/integrin/tight junction signaling, VEGF signaling |
| BMPR2 | BMP signaling, adherens junction signaling, PPARα/RXRα activation, TGF-β signaling | |
| BTG2 | Cell cycle regulation | |
| CDK6 | Cyclins and cell cycle regulation, HER-2 signaling, hereditary breast cancer signaling | |
| CDKN1A | See CDK6, role of BRCA1 in DNA damage response, p53 signaling, PI3K/AKT signaling, PTEN signaling | |
| CFL2 | Actin cytoskeleton signaling, role of tissue factor in cancer | |
| E2F1 | Breast cancer regulation by Stathmin1, cyclins and cell cycle regulation, hereditary breast cancer signaling, p53 signaling, role of BRCA1 in DNA damage response | |
| FAS(LG) | Apoptosis, p38 MAPK signaling, p53 signaling, PEDF signaling, PTEN signaling | |
| FGF1 | Actin cytoskeleton signaling, clathrin-mediated endocytosis signaling, epithelial adherens junction signaling, FGF signaling, regulation of the epithelial-mesenchymal transition pathway | |
| JAG1 | Notch signaling, regulation of EMT pathway | |
| PIK3R1 | Actin cytoskeleton signaling, breast cancer regulation by Stathmin1, HER-2 signaling in breast cancer, hereditary breast cancer signaling,p53 signaling, PTEN signaling, regulation of EMT pathway, VEGF signaling | |
| PTEN | Adherens junction signaling, ErbB signaling, hereditary breast cancer signaling, integrin signaling, p53 signaling, PI3K/AKT signaling, PTEN signaling, role of tissue factor in cancer, tight junction signaling | |
| SERPINB5 | p53 signaling | |
| TGFBR2 | Adherens junction signaling, glucocorticoid receptor signaling, p38 MAPK signaling, PPARα/RXRα activation, TGF-β signaling, tight junction signaling, Wnt/β-catenin signaling | |
| TNF | Apoptosis, glucocorticoid receptor signaling, p38 MAPK signaling, PPAR signaling, tight junction signaling | |
| hsa-miR-210 | ACVR1B | Adherens junction signaling, PPARα/RXRα activation, TGF-β signaling, Wnt/β-catenin signaling |
| E2F3 | Breast cancer regulation by Stathmin1, cyclins and cell cycle regulation, estrogen-mediated S-phase entry, role of BRCA1 in DNA damage response | |
| FGFRL1 | FGF signaling, NF-κB signaling, PTEN signaling, regulation of EMT pathway | |
| PTPN1 | Caveolar-mediated endocytosis signaling, insulin receptor signaling, JAK/Stat signaling, protein kinase A signaling | |
| hsa-miR-378a-5p | SUFU | Molecular mechanisms of cancer |
All p < 0.05, using IPA.