Figure 4. Adoptive transfer of tumor-specific CD8⁺ T cells eliminates established WT-AB1 malignant mesothelioma.

A. Time schedule of the adoptive transfer of CD8⁺ T cells. SCID mice were pre-inoculated s.c. with 5 × 10⁵ WT-AB1 cells, and tumors were left to grow for 7 days to reach approximately 5 mm in length. Cyclophosphamide (Cy) was injected intraperitoneally (150 mg/kg) one day before the adoptive transfer of CD8⁺ T cells. 2 × 10⁶ of isolated CD8⁺ T cells in 100 μl PBS were then injected via the tail vein into each mouse. B. Tumor growth was assessed by BLI overtime. CD8⁺ T cells of groups I, II and III were adoptively transferred i.v. into SCID mice of groups I’, II’ and III’, correspondingly. Intravenous transfer of CD8⁺ T cells of group I significantly eliminated established WT-AB1 tumor in SCID mice of group I’ (n = 5). Data represent mean ± s.e.m. *P = 0.0194. C. Representative BLI of WT-AB1 tumor growth in three groups of SCID mice. Images were acquired at the indicated time points after WT-AB1 tumor inoculation.