Skip to main content
. 2015 Sep 28;6(32):32439–32455. doi: 10.18632/oncotarget.5857

Figure 10. Bortezomib-mediated effect on T cell effector molecules is linked to Notch signaling.

Figure 10

Lymph node cells from WT Balb/c mice established with 14-day subcutaneous 4T1HA tumors were pre-treated with GSI (500 nM) for 1 h, followed by BZB (10 nM) treatment for 4 h. Data show expression of Hes1 and Hey1 mRNA A. as mean ± S.E.M; n = 12 pooled from 3 independent experiments; @p < 0.05, vehicle versus GSI; #p < 0.05, GSI versus BZB + GSI (ANOVA, one-way). Expression of mRNA for Hes1 and Hey1 B. and effector molecules perforin, granzyme B and eomesodermin C. in purified CD8+ T cells (from the pooled splenocytes and LN cells of WT Balb/c mice) pre-treated with GSI (500 nM) for 1 h and activated with CD3 and CD28 antibodies (1 μg/ml) for 24 h, followed by BZB (10 nM) treatment for 4 h. Data are expressed as mean ± S.E.M; n = 3; *p < 0.05, vehicle versus GSI; @p < 0.05, vehicle versus activation; #p < 0.05, activation versus activation + GSI; and $p<0.05, activation + GSI versus activation + GSI + BZB (ANOVA, one-way). Statistical analysis was based on three individual experiments.