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. 2015 Sep 28;6(32):32439–32455. doi: 10.18632/oncotarget.5857

Figure 9. Bortezomib stimulates the expression of Notch genes via NFκB activation.

Figure 9

CD8+ T cells purified from pooled cells of spleen and LN from naïve Balb/c mice were treated with 10 or 20 nM BZB for 4 h. A. Total and phosphorylated forms of p65 analyzed in the nuclear and cytoplasmic extracts, and B. total and phosphorylated forms of IKK and IκBα analyzed in whole cell preparations of BZB-treated CD8+ T cells by Western blots. The values represent the densitometric quantification of the immunoblots. GAPDH was used as loading control. Expression of Hes1 and Hey1 mRNA in CD8+ T cells stimulated in vitro using CD3 and CD28 antibodies (1 μg/ml, 24 h) C., or naïve without stimulation D., followed by treatment with BZB (10 nM, 4 h) along with NFκB inhibitor Bay-11-7082 (5 μM). Data are expressed as mean ± S.E.M; n = 3. *p < 0.05, vehicle versus activation or Bay11 versus Bay11 + BZB; @p < 0.05, activation (ACT) versus ACT + Bay11; #p < 0.05, ACT + Bay11 versus ACT + Bay11 + BZB (ANOVA, one-way). Statistical analysis was based on one representative experiment out of three individual experiments.