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. 2015 Oct 2;6(32):32468–32483. doi: 10.18632/oncotarget.5957

Figure 1. Piperine enhances mTORC1 activation in the presence of amino acids.

Figure 1

A.-F. Western blotting showing p-p70S6K levels in HeLa cells that were first starved in Earle's Balanced Salt Solution (EBSS, with glucose) for 50 min A. or Krebs-Ringer bicarbonate buffer (KRBB) for 50 min C. or KRBB for 3 h E., followed by incubation in indicated media (serum-free) for indicated time periods. Gln (L-glutamine, 2 mM), Leu (L-leucine, 0.8 mM) and/or piperine (40 μM) were added in EBSS A. or KRBB C., E.. Glc, glucose; NG, no glucose. Quantification of p-p70S6K relative to total p70S6K is shown in B., D. and F. (n = 3). The significance was analyzed by Student's t-test. *P < 0.05; **P < 0.01; ***P < 0.001.