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. 2015 Sep 28;6(32):32484–32493. doi: 10.18632/oncotarget.5398

Figure 1. Tregs, CD8+ T cells and PD-L1+ myeloid cells in the paracortical T cell area, peri-tumoral and tumor area in metastatic lymph nodes.

Figure 1

A. H&E staining of a representative tumor-positive lymph node (LN+) showing the peri-tumoral and tumor area. Triple immunofluorescence staining of FoxP3 (green), HLA-DR (red) and CD8 (blue) of a B. LN+ (sequential section to the one shown in (A), magnification 100x, scale bar is 20 μm) showing the presence of Tregs, CD8+ T cells and HLA-DR+ cells in C. the paracortical T cell area, D. the peri-tumoral area, and E. the tumor area in a LN+. Nuclei are counterstained with DAPI (grey) (magnification 200x, scale bar 20 μm). In a sequential section, also PD-L1+cells (in brown) were present in F. T cell area, G. peri-tumoral area, and H. tumor area. Nuclei are counterstained with Hematoxylin (blue) (magnification 200x, scale bar is 20 μm), more I. Tregs, J. CD8+ T cells and K. PD-L1+ cells were observed in the peri-tumoral areas compared to the metastatic tumor fields. In (H), arrow heads indicate PD-L1+ infiltrating cells among the weakly positive tumor cells. In (I) and (J), the overall P value was calculated with One-way ANOVA Friedman test, and Dunn's post hoc test was used to compare individual groups. The Fisher Exact Test was used in (K) *P = 0.01 to 0.05 and **P = 0.001 to 0.01.