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. 2015 Aug 3;6(32):32634–32645. doi: 10.18632/oncotarget.4728

Figure 4. Mammary epithelium cells-induced protrusions depend on microtubule dynamics.

Figure 4

MDA-MB-231 cells were incubated with MCF10A CM or filtered CM with a cutoff molecular weight of 100 kDA supplied with 5 μg/ml purified laminin in the present or absent of nocodazole (125 nM) and taxol (500 nM). A–E. Confocal image of the main protrusion extended from MDA-MB-231. Cells were stained for α-tubulin (red) and F-actin (green); nuclei were visualized with Hoechst 33342 (blue). Scale bar, 20 μm. F. Mean protrusive length and G. total number of invaded MDA-MB-231 cells through the Boyden chamber coated with a thick layer of matrigel. ****:P < 0.0001; **:P < 0.01.