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. 2015 Oct 5;6(32):32790–32804. doi: 10.18632/oncotarget.5411

Figure 3. Triptolide and celastrol synergistically induce G2/M cell cycle arrest in H1299 and H157 cancer cells.

Figure 3

H1299 cells was exposed to triptolide (30 nM), celastrol (0.6 μM) or a combination of both (triptolide 30 nM + celastrol 0.6 μM), and H157 cells was exposed to triptolide (15 nM), celastrol (1.6 μM) or a combination of both (triptolide 15 nM + celastrol 1.6 μM) for 48 h. The distribution of cell cycle was detected by FCM with PI staining. The percentages of subG1, G1/G0, S, G2/M phase were calculated using ModFit LT 3.0 software. The protein expression was examined by Western blot after lysing cells, and β-actin was used as loading control. The representative charts A. quantified results B. and Western blot results C. of three independent experiments were shown. *P < 0.05 vs. corresponding control.