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. 2015 Oct 5;6(32):32790–32804. doi: 10.18632/oncotarget.5411

Figure 5. ROS is critical for the synergistic anticancer effects of triptolide and celastrol.

Figure 5

H1299 and H157 cells were treated similarly as described in Figure 3 for 24 h, stained with DHE and photographed under fluorescent microscope. The representative micrographs A. and quantified results B. of three independent experiments were shown. Cells were co-treated with triptolide and celastrol for 24 h in the present or absent of 3 mM NAC pretreatment for 1 h. The apoptosis was detected by FCM with Annexin V/PI staining. The proportions of Annexin V+/PI− and Annexin V+/PI+ cells indicated the early and late stage of apoptosis. The representative charts C. and quantified results D. of three independent experiments were shown. *P < 0.05 and **P < 0.01 vs. corresponding control.