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. 2015 Sep 21;6(32):32902–32913. doi: 10.18632/oncotarget.5361

Figure 2. IL13Rα2 promotes proliferation, invasion, migration and anoikis resistance in lung cancer cells.

Figure 2

Figure 2

A. The protein expression level of IL13Rα2 in lung cancer cells and normal lung epithelial cells were measured using western blotting assay. B. HTB-57 and A549 cells were transfected with control shRNA (shCTRL) or IL13Rα2 shRNA (shIL13Rα2). NCI-H3255 and PC9 cells were transfected with IL13Rα2 or control vector. Transfection efficiency was evaluated by RT-PCR analysis. C. Proliferation assays were performed by CCK-8 kit in IL13Rα2-transfected and IL13Rα2-silenced lung cancer cells pretreated with or without IL-13. *P < 0.05 was considered statistically significant when compared with control cells. D-E. Transwell migration and invasion assays were conducted. HTB-57 and A549 cells transfected with shIL13Rα2 or shCTRL were treated in presence of medium alone or with IL-13. The cell numbers at the bottom are the means ± SD of the counts in eight random fields for three independent experiments. *P < 0.05. F. IL13Rα2 transfected NCI-H3255 cells and IL13Rα2 silenced HTB-57 cells were cultured on tissue culture plates (attach) or on poly-HEMA pre-coated plates (suspension) in presence of IL-13. After 72 h, cells were collected, stained with PE Annexin V and analyzed by flow cytometry. Experiments were done in duplicate. *P < 0.05.