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. 2015 Sep 26;6(32):32966–32979. doi: 10.18632/oncotarget.5435

Figure 1. OVA intestinal allergy in WT mice induces the production of IL-4 and accumulation of HDC-expressed CD11b+Gr1+ cells.

Figure 1

WT mice (n = 5/group) were immunized with OVA (30 ug i.g. and 50 ug i.p.) or PBS. A. Serum IL-4 levels were quantified by ELISA (Left); Intestinal IL-4Ra expression was exanimated by qRT-PCR (Right). B. Representative flow plots of circulating HDC-EGFP+ in blood CD11b+Gr1+ cells (Left). Bar graph (Right) illustrates the proportions of cell populations with statistics. C. Imagines show EGFP+ cells from OVA treated and PBS controls small intestine frozen sections (Left). EGFP+ cells were enumerated in each observed microscopic field (Right). Expression of Hdc mRNA D. and IL-4Rα E. from sorted small intestine CD11b+Gr1+ cells. F. Representative images of small intestine and colon immunofluorescence staining for tryptase+ MCs in HDC−/− mice treated with either OVA or PBS (Left). Tryptase+ MCs were counted per microscopic field (Right). Data are representative of at least two independent experiments.