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A. Co-culture of BMMCs and splenocytes from HDC−/− mice in the presence of OVA323–339 peptide. 96 h after culture, CD11b+Gr1+ cells were sorted, expressions of Cox-2, Arg-1 and Ki67 were determined by qRT-PCR. B. Supernatant IL-17 was determined by ELISA. C. Representative flow plots show the frequency of CD3+CD8+ cytotoxic T cell in tumor margin colon tissue (n = 3). Data are representative from 3 independent experiments.
