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. 2015 Sep 5;6(32):33091–33105. doi: 10.18632/oncotarget.5173

Figure 5. Sur8 interacts with the p110α subunit of PI3K in an EGF- and oncogenic Ras-dependent manner.

Figure 5

A. HEK293 cells were transfected with the indicated plasmids or siRNAs (siGFP or siSur8 #1 and #2), and WCLs were immunoblotted against the indicated proteins. B. WCLs of HEK293 cells were immunoprecipitated with either control IgG or Sur8 antibody and immunoblotting was performed against the indicated proteins. C–E. HEK293 cells were transfected with the indicated plasmids, treated or non-treated with EGF and WCLs were immunoprecipitated with either anti-Sur8 (C) or -p110α (D, E) antibody and immunoblottings were performed against the indicated proteins. F. GST and GST-Sur8 were incubated with His-p110α recombinant protein (3 μg each) for 1 hour at 4°C. Glutathione agarose beads were used to pull down p110α as described in Materials and methods. Immunoblotting was performed using anti-Sur8 or -p110α antibody. Cells in figures (C) and (D) were treated with EGF for 10 minutes.