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. 2015 Aug 12;6(32):33237–33252. doi: 10.18632/oncotarget.4893

Figure 5. Knocking down miR-20a expression upregulates TβRII and inhibits tumorigenecity in vivo.

Figure 5

A. Human lung cancer cells ACC-LC-176 with miR-20a knockdown and control vector cells were subcantaneously injected into nude mice. Tumor length (L) and width (W) were measured every three days. Tumor volumes were calculated using the equation V = 0.5LW2. Growth curves for tumors were plotted as the mean volume ± SD of tumors of mice from each group. B, C, D. and E. Expression of miR-20a, TβRII, p21CIP1 and PAI-1 in tumor xenografts were analyzed by QRT-PCR. *P < 0.05, vs. vector group. F. Immunohistochemical analyses for the expression of p21CIP1 and P-Smad2 in xenograft tumors were performed. Representative pictures are shown (X 400).