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. 2015 Sep 5;6(32):33397–33409. doi: 10.18632/oncotarget.5179

Figure 1. BRD4 and BRD2 are expressed in UM cells.

Figure 1

A. Total RNA was extracted from eight cell lines with the indicated mutational status, and qPCR was performed using gene-specific primers for BRD4 and BRD2. Values were normalized with GAPDH as housekeeping gene using the ΔΔCT method. Values are relative to mRNA levels of 92.1 cells set at 1. Each experiment was performed in triplicates. Bars, mean ± sd. B. BRD4 is active in UM cells. Silencing of BRD4 down-regulates c-Myc expression in Gnaq mutant- and WT cell lines, as detected by immunoblotting. C. Cells were treated with 500 nM JQ1 for 24 hours and cell lysates were analyzed for c-Myc expression and tubulin as loading control.