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. 2015 Sep 5;6(32):33397–33409. doi: 10.18632/oncotarget.5179

Figure 2. JQ1 induces cell cycle arrest and apoptosis in UM cells.

Figure 2

A. JQ1 reduces viability of a panel of UM cell lines with the indicated mutational status. The cell lines were exposed to 2-fold serial dilutions 2000–100 nM of JQ1 in triplicates for 4 days, and viability was normalized to DMSO-treated cells. Data points are mean ± sd. B. Gnaq-mutant and WT cell lines were treated with DMSO or 500 nM JQ1 over time up to 72 hours. The cells were stained with propidium iodide (PI) and analyzed for cell cycle distribution by flow cytometry. Sub-G1 populations were 19.8% and 19.2% for 92.1 and Omm1.3 cells, respectively. C. UM cells were treated with 500 nM JQ1 for 48 hours, then incubated with YO-PRO dye (green) and PI (red). Bars report the percent of cells with the sum of green and red fluorescence for each condition in triplicates ± sd. D. The same cell lines (Gnaq-mutant top panel; WT, bottom panel) were treated over time with JQ1 and lysed for Western blot analysis, showing induction of apoptosis by PARP cleavage.