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. 2015 Oct 2;6(32):33410–33425. doi: 10.18632/oncotarget.5418

Figure 4. TOPK inhibitor exhibits anti-leukemia activity in FLT3 mutated AML blasts and in MV4-11 murine model.

Figure 4

A. Blasts obtained from three AML patients with FLT3-ITD mutations and relapsed following AC220 clinical trial, cells were treated with increasing concentration of OTS514 and viability assay was performed 48 hours later. B. Apoptosis was assessed by annexin and PI staining in blasts obtained from three AML patients with FLT3-ITD mutation C. Blasts from AML patient with FLT3-ITD mutation were treated with 20 and 40 nM of OTS514 and apoptotic cells were assessed by annexin and PI staining 5 days later. D. Image of spleens obtained from vehicle and OTS514 treated mice. E. Quantitative analysis of spleen weights (Mean ± SEM). F. Mean relative mouse body weight ± SD (N = 6 mice per group) in comparison with the body weight just before the administration. G. Survival analysis of OTS514-treated leukemic mice (N = 6) compared with the vehicle-treated controls (N = 6) (P < 0.001).