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. 2015 Sep 10;6(32):33636–33647. doi: 10.18632/oncotarget.5602

Figure 2. SIP1 inhibition attenuates GADD45G-induced tumor cell senescence in vitro.

Figure 2

(A-B) Tet-on-GADD45G-Sk-Hep1 A. and Tet-on-GADD45G-SMMC-7721 cells (B) were transfected with siRNA targeting SIP1 (siSIP1) or with control siRNA ( siCon), then cultured for 3 days with or without GADD45G induction. Representative images of SA-β-gal staining (left panel) and the percentages of positive cells (right panel) are shown. Data shown are mean ± SD from three independent experiments (***P < 0.001). SD, standard deviation. (C-D) Tet-on-GADD45G-Sk-Hep1 (C) and Tet-on-GADD45G-SMMC-7721 cells (D) were transfected with the indicated siRNAs, then cultured for 3 days with or without GADD45G induction. The efficiency of the siRNA for the downregulation of SIP1 protein was confirmed by Western blot. (E-F) hTERT mRNA levels were measured in Tet-on-GADD45G-Sk-Hep1 (E) and Tet-on-GADD45G-SMMC-7721 (F) cells with the indicated treatments. Data shown are mean ± SD from three independent experiments (*P < 0.05, **P < 0.01). SD, standard deviation. (G-H) Tet-on-GADD45G-Sk-Hep1 (G) and Tet-on-GADD45G-SMMC-7721 cells (H) with the indicated treatments were harvested for analysis of the percentage of cells in each cell cycle phase by fluorescence-activated cell sorting. Data shown are mean ± SD from three independent experiments. SD, standard deviation.