Figure 2. Rv2299c injection results in enhanced anti-tumor activity against MC-38 colon cancer.

A. Schematic experimental schedule of in vivo animal vaccinations with Rv2299c. Experiments consisted of five mice per group. B. Mice treated with 5 and 10 μg of Rv2299c showed significantly greater inhibition of tumor growth compared to those receiving 1 μg of Rv2299c or the PBS control (*P < 0.05), as measured by the monitoring tumor volume. C, D. Mice in the 5- and 10-μg Rv2299c treatment groups had an increased proportion of CD4⁺ T cells and CD8⁺ T cells among splenocytes compared to the 1-μg Rv2299c or PBS control group, as measured by flow cytometry in the left panel and compared by quantitated bar graphs in the right panel. Data are representative of more than 3 experiments.