Figure 5. MUC4 regulates the expression of netrin-1 in PDAC.
A. Colo-357 and Capan-1 cells were treated with Scr and shMUC4, MUC4 and netrin-1 expressions were determined by immunofluorescence analysis. Alexa Fluor 488 (green) for MUC4, Alexa Fluor 594 (red) for netrin-1 andDAPI (blue) for nuclear staining. Original magnification, 200×; scale bar = 100 μm. B. MUC4 and netrin-1 expressions in nude mice xenografts were detected by immunohistochemicalanalysis. Original magnification, 200×; scale bar = 100 μm. C. Western blot analysis of MUC4 and netrin-1 expressions in Scr-treated and shMUC4-treated cells. D. Linear regression analysis was used to determine the correlation between the MUC4 and netrin-1 mRNA expression levels in human PDAC tissues. R2 = 0.48, p < 0.01, n = 82.