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. 2015 Sep 15;6(32):33805–33822. doi: 10.18632/oncotarget.5668

Figure 6. Exogenous overexpression of netrin-1 in shMUC4-treated cells restores their NI capability.

Figure 6

A. Western blot analysis of MUC4 and netrin-1 expressions in Colo-357 Scr, Colo-357 shMUC4, Colo-357 shMUC4/netrin-1, Capan-1 Scr, Capan-1 shMUC4 and Capan-1 shMUC4/netrin-1 cells. (B, C)The migratory capabilities wereevaluated by wound healing assays and Transwell migration assays, B. the average area covered by migrating cells is presented, C. the number of migrated cells was quantified. D. The invasive capabilities were investigated by Transwell invasion assays. The number of invasive cells was quantified. (E-G) The interaction between tumor cells and neurites was evaluated by the DRG-PDAC cell co-culture assay, E. the accumulated distance travelled by the cancer cells was calculated, F. the travelling velocity of cancer cells was calculated, G. the average area covered by the neurites growing out from the DRG was quantified. H. The NI capabilities in the mouse NI model were showed by Scatter plot. Differences were analysed by the Mann-Whitney U-test. The pooled data are presented as mean ± SEM. *p < 0.05, **p < 0.01.