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. 2015 Sep 16;6(32):33867–33877. doi: 10.18632/oncotarget.5293

Figure 3. Impact of EGFRvIII expression on EGFR downstream signaling, proliferation and clonogenicity.

Figure 3

A. Effect of EGFRvIII expression on EGFR signaling as determined by Western blot analysis using antibodies against (p)EGFR (Y1173), (p)AKT (T308) and (p)ERK1/2 (T202/Y204). U87MG cell served as a negative control cell line. B. Morphology of EGFRvIII−/+ DKMG and BS153 cells (phase-contrast microscopy, 100× magnification). C. Proliferation of EGFRvIII− and EGFRvIII+ DKMG and BS153 cells. The cell number was determind for up to 8 days. D. Relative clonogenicity of EGFRvIII−/+ DKMG and BS153 cells as determined by colony forming assay (pre-plating).