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. 2015 Oct 14;6(37):39960–39968. doi: 10.18632/oncotarget.5662

Figure 1. Influence of wall shear stress on nanoparticle binding to endothelium under flow.

Figure 1

A. Scheme of shear stress determinants in nanocarrier binding to endothelium. B–D. Binding of mAb-coated nanoparticles or mAb to endothelium on tissue slides was studied using a microfluidic chamber and image-based fluorimetry. Endothelial binding of self-coated nanoparticles (B) and CD146(LSEC)-Microbeads (C) decreased with increasing shear stress, whereas no significant difference of ME-9F1 mAb binding was found (D) E. Representative immunofluorescence imaging of nanoparticle and mAb binding to endothelium on microfluidic tissue slides. Fluorescence labeling of NP was performed by PE-conjugated secondary antibody. Scale bar (50 μm) as indicated.