Figure 2. SIVmac239 Vif efficiently counteracts huA3B-mediated restriction of HIV-1.

A. Bar graph depicting the infectivity (measured as % infected CEM-GFP reporter) of Vif-deficient HIV-1_IIIB complemented with vector (grey bars), HIV-1_IIIB Vif (blue bars), or SIVmac239 Vif (red bars); produced in the presence of vector control, huA3B, rhA3B or huA3G (n = 3; mean and SD shown). Asterisks indicate level of significance, compared to the no Vif condition (***p < 0.001, as determined by one-way ANOVA). B. Representative immunoblots for each infection condition are shown beneath each histogram bar. Purified viral particles were blotted for HA to detect A3 and for p24 (Gag) as a loading control. Producer cell lysates were blotted for HA to detect A3, for MYC to detect Vif, and for Tubulin (TUB) as a loading control. The migration positions of molecular weight standards are indicated next to the anti-MYC (Vif) panels.