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. 2015 Oct 31;6(37):39969–39979. doi: 10.18632/oncotarget.5483

Figure 5. SIVmac239 Vif degrades endogenous huA3B in cancer cells.

Figure 5

A. Representative immunoblots of HCC1569, JSQ3, and OVCAR5 cells expressing empty vector, HIV-1IIIB Vif, or SIVmac239 Vif. Cell lysates were blotted for endogenous huA3B, for MYC to detect Vif, and for tubulin (TUB) as a loading control. B. Quantification of endogenous huA3B in cancer cells expressing empty vector, HIV-1IIIB Vif, or SIVmac239 Vif. A3B levels were normalized to Tubulin, and the amount of A3B in the presence of vector, for each cell line, was set at 1 (n = 3, mean and SD shown). Asterisks indicate level of significance, compared to vector control (**p < 0.01; ***p < 0.001, as determined by two-way ANOVA). C. Representative immunoblot of OVCAR5 cells demonstrating that SIVmac239 Vif-mediated degradation of endogenous huA3B is dependent on the SLQ motif and that this degradation is inhibited in the presence of MG132 (5 μM, 16 hours). Cell lysates were blotted for endogenous huA3B, for MYC to detect Vif, and for tubulin (TUB) as a loading control.