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. 2015 Oct 19;6(37):40005–40025. doi: 10.18632/oncotarget.5552

Figure 6. Induction of a pro-inflammatory secretory phenotype after CPT treatment of SKOV3 cells.

Figure 6

A–E. RT-qPCR expression analysis of the indicated pro-inflammatory marker genes at the indicated times of CPT treatment (sample size: n ≥ 3). FC = 1 (day 0) corresponds to the following Ct values: IL1B: 31.5; IL8: 32; CCL20: 32; CXCL1: 22; SERPINE1: 26. F. Flow cytometry analysis of intracellular IL-1β expression after 0, 2 or 21 weeks of CPT treatment (sample size: n = 3). Values represent the fold change (FC) in the number of positive cells relative to small cells on day 0 (normalized to 1). Cells gated for small cells (forward and sideward scatter as untreated SKOV3 cells) and larger cells (forward and sideward scatter larger than for SKOV3 cells). G. Immunoblot analysis of phosphorylated STAT3 in untreated human macrophage cells (negative control), after LPS treatment (positive control), or after an exposure to supernatant from SKOV3 cells treated with CPT for 0, 5 days, 14 days or 21 weeks.