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. 2015 Oct 12;6(37):40112–40126. doi: 10.18632/oncotarget.5556

Figure 5. SIX1 regulates expression of GATA2 and GATA3.

Figure 5

A. RQ-PCR analysis of GATA2 after siRNA-mediated knockdown of SIX1 in L-428 cells (left), demonstrating activation of GATA2 transcription by SIX1. Western blot analysis (left) confirmed reduced SIX1 expression after siRNA-mediated knockdown in L-428 cells. Quantification of GATA2 by RQ-PCR in HL cell lines (middle) and primary hematopoietic cells and tissues (right), demonstrating aberrant GATA2 expression in L-428 as compared to B-cells. B. RQ-PCR analysis of GATA3 after siRNA-mediated knockdown of SIX1 in L-428 cells (left), demonstrating suppression of GATA3 transcription by SIX1. Quantification of GATA3 by RQ-PCR in HL cell lines (middle) and primary hematopoietic cells and tissues (right), demonstrating GATA3 overexpression in L-428 as compared to B-cells.