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. 2015 Oct 12;6(37):40141–40157. doi: 10.18632/oncotarget.5653

Figure 6. MPN cells that persistently grow in the presence of JAK2 inhibitors are still sensitive to the combination of ruxolitinib and AZD1208.

Figure 6

A. Ruxolitinib persistent Uke1-R and BaF3-JAK2-V617F-R cells growing in 1 μM ruxolitinib were plated in 1 μM ruxolitinib and 0.1 or 0.5 μM AZD1208, as indicated. Relative viable cells were determined by MTS assay after 72 hours. B. Ruxolitinib persistent BaF3-JAK2-V617F-R cells were cultured in 1 μM ruxolitinib alone or with 0.25 or 0.5 μM AZD1208 and total viable cells were determined over time by trypan blue exclusion. C. BaF3-JAK2-V617F-R cells growing in 1 μM ruxolitinib were treated with ruxolitinib alone, 0.25 μM AZD1208, or the combination of the two drugs. Cell viability after two and four days was determined by trypan blue exclusion. D. Apoptosis in BaF3-JAK2-V617F-R cells treated with 1 μM ruxolitinib, 0.25 μM AZD1208, or a combination of the two drugs was detected with annexin V staining and flow cytometry after 24, 48, and 72 hours. Error bars indicate standard deviation.