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. 2015 Oct 2;6(37):40247–40254. doi: 10.18632/oncotarget.5377

Figure 2. Analysis of ERβ and p65 localization and expression in prostate cells and tissues.

Figure 2

A. Control and ERβ-depleted PNT1a cells and B. Ventral prostates from 10 month old wild-type and BERKO mice were stained with a p65 antibody and DAPI to visualize nuclei, and analyzed by immunofluorescence microscopy. Scale bar: 50 μm. C. The expression of ERβ, HIF-1α and p65 was compared in PNT1a and PC3-M cells by immunoblotting. D. The expression of p65 was compared in control (shGFP) and PHD2-ablated PNT1a cells (shPHD2#1 and shPHD2#2) by immunoblotting. E. An inverse correlation between ERβ and p65 in a cohort of 82 prostate tumors was determined from analysis of the cBioportal database.