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. 2015 Sep 10;6(37):40268–40282. doi: 10.18632/oncotarget.5592

Figure 5. Inhibition of autophagy suppresses cell survival and promotes crizotinib-induced apoptosis in vitro.

Figure 5

A. & B. Cells were treated with the indicated concentration of crizotinib or DMSO in the presence or absence of CQ (5 μM) or 3-MA (1 mM) for 72 h. Cell viability was measured by MTS assay. C. SPC-A1 cells were treated with crizotinib alone or in combination of CQ or 3-MA before staining with annexin V (AV) and propidium iodide (PI), and the apoptotic rates were determined by flow cytometry. D. & E. Cells were transfected with shRNA against Beclin-1 (shBeclin1) or scramble (UNR) for 24 h and then treated with crizotinib for 48 h. Cell viability was measured by MTS assay. F. SPC-A1 cells were transfected with shRNA against Beclin-1 for 24 h and then treated with crizotinib for 48 h before analysis; the apoptotic rates were determined by staining with AV & PI followed by flow cytometry. Each dot or column represents three individual experiments. Data are expressed as the mean ± SD, and analyzed by 2 way ANOVA. *** P<0.001.