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. 2015 Sep 25;6(37):40370–40384. doi: 10.18632/oncotarget.5458

Figure 3. Analysis of the expression of caldesmon (CAD) variants in bladder cancer (BC) cell lines.

Figure 3

A. Schematic summary of the CAD isoforms. The identical sequences in all CAD isoforms and the central repeating domain specific to H-CAD are indicated by solid bars and open bar, respectively. The short amino acid sequences specific to CAD variants are shown. Primers used in PCR analysis are indicated by arrows and the sizes of amplified PCR products in each transcript variant are described. B. Agarose gel electrophoresis of CAD RT-PCR products from BC cell lines. RT-PCR was conducted using first-strand cDNA from each BC cell line with the indicated primer set. HeLa S3 (HeLa) and WI-38 (WI38) cells were used as positive controls. M: 100 bp DNA size marker.