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A. Western blotting of ISCs by Erk1/2, P-Erk1/2, Akt, P-Akt, Smad2/3, P-Smad2/3, Smad7, TGF-β antibody. Data are expressed as mean ± SE (n = 3), * P < 0.05, ** P < 0.01. B. Western blotting of db/db ISCs treated with rhReg1, U0126 (10uM), LY-294002 (10uM), SB431542 (10uM), sh-Reg1, sh-EXLT3 and sh-EXTL3+Reg1 by Erk1/2, P-Erk1/2, Akt, P-Akt, Smad2/3, P-Smad2/3 and Smad7 antibody.
