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. 2015 Oct 26;6(35):37316–37334. doi: 10.18632/oncotarget.5686

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Copyright: © 2015 Lee et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. Cells were grown in 3D matrigel culture for two weeks and acini were fixed in 4% paraformaldehyde and pictures of live acini were taken by Olympus IX51. B. Cells were processed for immuno fluorescence with the indicated markers to calculate. C. the volume of acini by using the Imaris software (log mean ± S.E.M) D. percentage of acini displaying protrusions was generated by manually scoring out cells detached from acini. E. The invasion assay was performed on cells by using BD Biocoat matrigel invasion chambers.

A. Cells were grown in 3D matrigel culture for two weeks and acini were fixed in 4% paraformaldehyde and pictures of live acini were taken by Olympus IX51. B. Cells were processed for immuno fluorescence with the indicated markers to calculate. C. the volume of acini by using the Imaris software (log mean ± S.E.M) D. percentage of acini displaying protrusions was generated by manually scoring out cells detached from acini. E. The invasion assay was performed on cells by using BD Biocoat matrigel invasion chambers.