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. 2015 Oct 19;6(35):37349–37366. doi: 10.18632/oncotarget.5736

Figure 6. Mitochondrial EGFR induces mitochondria fission independent of its phosphorylation status.

Figure 6

A. CL1–0 cells were transduced with control or mitEGFR mutant plasmids. And the cells were subjected into immunoblotting for confirming the expression and phosphorylation of EGFR. B. Mitochondria length per cell was estimated by the high-content microscopic system in CL1–0 transducd with mitEGFR mutant constructs. The right panel showed that the cell populations were categorized into the tubular and fragmented morphology, according to the mitochondrial length and morphology. The cell number of 6 groups was 455, 425, 427, 428, 451 and 379 respectively. C. CL1–0 cells were co-transduced with control or mitEGFR mutant plasmids, and then reseeded in the transwells in triplicate. After 22 h, migrated cells were quantified, and the relative percentage of migrated cells in each group to that in the control group is presented. In all the panels mean ± s.d. is shown, *P < 0.05, **P < 0.001 and ***P < 0.0001 by Student's t-test.