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. 2015 Oct 14;6(35):37737–37749. doi: 10.18632/oncotarget.5648

Figure 3. LLC-bearing mice harboring JDP2-deficient bone marrow exhibit reduced metastasis.

Figure 3

A. Wild-type C57B1/6 mice were transplanted with BMDCs from either wild-type (WT) or JDP2−/− mice. The chimeric mice were then subcutaneously implanted with 0.5 × 106 LLC cells, and tumor volume was monitored over time. B–E. At end point, tumors and lungs were excised. Lungs were embedded in paraffin, sectioned, and subsequently stained with H&E. Arrows indicate metastatic lesions. Scale bars = 2000 μm. Small micrographs are 2X magnification (B) The number of pulmonary metastatic lesions per field was quantified (C) Tumors were prepared as single cell suspensions and the percentage of mature (CD11b+Ly6C(low)Ly6G+) and immature (CD11b+Ly6C(high)Ly6G+) neutrophils (D), as well as the total number of neutrophils per 10,000 cells (E), were determined by flow cytometry. *, p < 0.05; ***p < 0.001 of a two-tailed t-test.