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. 2015 Oct 8;6(35):37782–37791. doi: 10.18632/oncotarget.5622

Figure 2. The effect of R11-p53C on p53-regulated gene expression and growth inhibition.

Figure 2

A. Dose-dependent induction of G1 cell cycle arrest by R11-p53C in T24 cells. Results represent three different experiments, each of which was performed in triplicates. *P < 0.05. B. Total cellular RNA was collected from T24 cells exposed to 5 μM R11-p53C or R11-Con. then subjected to qRT-PCR for determining PUMA, p21, GADD45A and PIG3 at the indicated time. C. T24 cells were pre-treated with pifithrin-α 20 μM (or DMSO) before 5 μM R11-p53C or R11-Con treatment for 48 hrs then subjected to western blot analysis. D. T24 cells were pre-treated with pifithrin-α (20 μM) or DMSO before 5 μM R11-p53C or R11-Con treatment for 48 hrs then subjected to MTT assay. Data represent mean and standard deviation from three different experiments; each experiment was carried out in triplicates.