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. 2015 Oct 12;6(35):37792–37807. doi: 10.18632/oncotarget.6096

Figure 1. Reciprocal regulation of Crk and Abi1 genes in glioblastoma.

Figure 1

A. Bioinformatics meta-analysis of cancer genome sequencing data: A.-C. Oncomine database was used to analyze the genomic and transcriptomic variations in Crk and Abi1 genes by meta-analysis of indicated studies data. The result of the analysis is represented in box-whisker plots and copy numbers and mRNA expression values are presented on a log2 scale. The results indicate an upregulation of Crk gene expression A. downregulation of Abi1 gene expression B. suppression in copy number of Abi1 gene C. (P < 0.003). D.-E. SurvExpress analysis using TCGA Brain 2013 data to assess survival outcomes in risk groups as compared with gene expression profiles of Crk(D). and Abi1 (E). F. Western blot analysis validates bioinformatics analysis indicating a significant suppression in Abi1-Iso2 levels in T98G, HS683 and U87MG cells. G. Western blot analysis of patient GBM tissue and normal tissue samples: 18 normal and 32 GBM biopsied samples (obtained from Wenzhou University Medical Center) were immunoblotted with anti-EGFR, anti-CrkpY251, anti-Crk, or anti-Abi-Iso2 antibodies. H. Samples were normalized to the actin-loading controls and quantified by densitometric scanning (anti-EGFR = black; anti-pCrk251 = blue; anti-Crk = green; anti-Abi-Iso2 = red).