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. 2015 Oct 16;6(35):37852–37870. doi: 10.18632/oncotarget.5635

Figure 5. Ad-lnc-p21-MRE decreases the viability and stem-like traits of CSCs by suppressing β-catenin signaling.

Figure 5

A. ALDH+ CSCs were transduced with the adenoviruses at indicated MOIs for 7 days, or incubated together with a GSK-3 inhibitor CT99021 (3 μM). The cell viability was measured by CCK8 reagent. B. ALDH+ CSCs were infected with the adenoviral vectors (10 MOI), with or without CT99021 incubation (3 μM). The cell viability was determined by CCK8 assay kit at indicated time points. C. ALDH+ CSCs infected with the adenoviruses (10 MOIs) were cultured in stem cell medium for 14 days. CSCs infected with Ad-lnc-p21-MRE were further treated with CT99021(3 μM) or solvent. The numbers of spheres originating from single ALDH+ cells were quantified. The parental spheres were dissociated and secondary and tertiary sphere formation was also examined. All the data are presented as the mean ± SD of each group from triple replicates. *P < 0.05, **P < 0.01.