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. 2015 Sep 25;6(35):37906–37918. doi: 10.18632/oncotarget.5412

Figure 4. The loss of Usp22 results in a differentiation shift in the small intestine.

Figure 4

Reduction of Usp22 expression causes a slight increase in the number of olfactomedin 4 (OLFM4)-positive stem cells as revealed after in situ hybridization (wt/wt: n = 5; wt/lacZ: n = 4, p = 0.53; lacZ/lacZ: n = 5, p = 0.021). Immunohistochemistry shows an increase of differentiated cell types in the small intestine. The number of mucus-producing Goblet cells (Muc2) hardly varied in the crypts but was significantly increased in the villi (wt/wt: n = 5; wt/lacZ: n = 5, p = 0.69; lacZ/lacZ: n = 5, p = 0.023). A raised rate of enteroendocrine cells (CgA) was observed as well (wt/wt: n = 5; wt/lacZ: n = 5, p = 8.25.10–8; lacZ/lacZ: n = 5, p = 6.20.10–7). Paneth cells (Lyz) which are located at the bottom of the crypt show an increased presence in Usp22lacZ/lacZ animals (wt/wt: n = 3; wt/lacZ: n = 3, p = 0.15; lacZ/lacZ: n = 3, p = 0.017). Scale bar: 100 μm.