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. 2015 Oct 12;6(35):37965–37978. doi: 10.18632/oncotarget.5700

Figure 1. IL-6 expression was up-regulated by lapatinib in MDA-MB-231 TNBC cells.

Figure 1

A. Total RNA extracted from MDA-MB-231 (231) cells and their lapatinib-treated clones (231/Lap) was applied to examine the IL-6 mRNA level in both general RT-PCR (upper panel) and quantitative RT-PCR (lower panel) analysis. B. The whole cell lysates of 231 and their lapatinib-treated clones were harvested and subjected to western blot analysis to examine the protein expression of IL-6. C. The incubated media from 231 cells and 231/Lap cells were collected and subjected to ELISA for detection of IL-6 protein secretion. D. IL-6 expression in tumor sections of MDA-MB-231 xenograft mouse receiving lapatinib treatment was determined in immunohistochemical staining with specific anti-IL-6 antibody. E. The whole cell lysates of primary human breast cancer cells treated with or without lapatinib for indicated time were harvested and subjected to western blot analysis to examine IL-6 protein expression. F. The incubated media from primary human breast cancer cells from (E) were collected and subjected to enzyme-linked immunosorbent assay (ELISA) for detection of IL-6 protein secretion. Results were expressed as mean ± S.E.M of three independent experiments. **: p < 0.01; ***: p < 0.001; #: p < 0.05 as compared with control group.