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. 2015 Oct 12;6(35):37965–37978. doi: 10.18632/oncotarget.5700

Figure 5. MAPK/AP-1 axis mediates lapatinib-induced IL-6 gene transcription.

Figure 5

A–C. 231 or 231/Lap cells were transfected with wt (A) and/or AP-1 mutated (B) IL-6 promoter luciferase reporter gene for 2 days followed by treatment with MAPK pathway inhibitors (C), and then total lysates were prepared and subjected to luciferase activity assays. D. Total lysates from 231 and 231/Lap cells were prepared and subjected to chromatin immunoprecipitation assay with anti-c-Jun antibody to detect the DNA binding activity of c-Jun on IL-6 promoter. One percent of the precipitated chromatin was used to verify the equal sample loading (input). *: p < 0.05; **: p < 0.01; ***: p < 0.001 as compared with control group.