Figure 4. RNA-binding motif 4 [RBM4] and neuronal polypyrimidine tract-binding protein [nPTB] exerted opposite effects on the splicing patterns of the FGFR2 and PKM genes.

Total RNA extracted from A. paired colorectal cancer [CRC] tissues and B. CRC cell lines were analyzed using an RT-PCR with specific primer sets. C. HCT-8 and HCT-116 cells were transfected with overexpressing or targeting vectors or synthesized siRNA, followed by total RNA extraction. Splicing profiles of FGFR2 and PKM pre-mRNAs were analyzed as described in Materials and methods section. The bar graph shows relative levels of FGFR2 IIIc and PKM2 in three independent experiments using TotalLab Quant Software [*p < 0.05; **p < 0.01; ***p < 0.005].