Figure 7. Expression profiles of RNA-binding motif 4 [RBM4] and neuronal polypyrimidine tract-binding protein [nPTB] modulate the mitochondrial activity of colorectal cancer [CRC] cells.

A. HCT-8 and B. HCT-116 cells were transfected with a parallel set of expressing or targeting vectors for 24 h. The basal and maximal oxygen consumption rates and spare respiratory capacity were measured using an XF20 Bioanalyzer [n = 4]. C. The RBM4-regulated splicing cascade was closely correlated with the cancerous signatures of CRC cells. Elevated miR92a-2 reduced the RBM4 level, which relieved its repressive effect on nPTB expression. The imbalanced expression of nPTB respectively enhanced relative levels of the FGFR2 IIIc and PKM2 transcripts which modulated the progression and energy expenditure of CRC cells.