| Real-time PCR primer |
|
| miR-331-3p-F |
GCCCCTGGGCCTATCCTAGAA |
| U6-F |
AGAGCCTGTGGTGTCCG |
| U6-R |
CATCTTCAAAGCACTTCCC |
| β-actin-F |
GTGGATCAGCAAGCAGGAGT |
| β-actin-R |
TGTGTGGACTTGGGAGAGGA |
| ING5-F |
ACCAGAGGACGGAAGATAAG |
| ING5-R |
TGCACTTGCTGTAGGCGTTC |
| VHL-F |
ACATCGTCAGGTCGCTCTAC |
| VHL-R |
ATCTCCCATCCGTTGATGTG |
| AKAP11-F |
AGCTGGAGCAGTCTTGGTTT |
| AKAP11-R |
ACTTCGTCCTCATGCTCTTC |
| ERBB2-F |
ACCTGCTGAACTGGTGTATG |
| ERBB2-R |
TGACATGGTTGGGACTCTTG |
| RGS-F |
TGCCCATCAGAACAGTCAAG |
| RGS-R |
ACTGGGTCCTCAATGGAAAG |
| DOHH-F |
AGGCCTTCGATGACGATTC |
| DOHH-R |
TGTCTTGCAGCACGTCCAC |
| SPOP-F |
AGAGTCAACGGGCATATAGG |
| SPOP-R |
AGCTTGTCATCAGGGAGAAG |
| TRERF1-F |
ATGACCTCCAGCAAAGAGTG |
| TRERF1-R |
TTGGCCTCAAACAGAGAGTG |
| miR-331-3p over-expression primer |
|
| pTARGET-miR-331-3p-F |
CGCCGCTCGAGATAATATCCTAAACAAAGCA |
| pTARGET-miR-331-3p-R |
ACGCGTCGACTTTTAGGGCTAAGTTGCTTC |
| miR-331-3p promoter primer |
|
| pGL3-Basic-miR-331-3p-F |
CGGGGTACCAACCGCCTGACCAACATGGAG |
| pGL3-Basic-miR-331-3p-R |
CCGCTCGAGTCCCTGGGACCATACCTAGAAC |
| ING5 3′UTR primer |
|
| pGL3-Control-ING5-WT-F |
AGCTCTAGAAGACCCATCTCTTGGATTG |
| pGL3-Control-ING5-WT-R |
AGCTCTAGATCCAACACAATCTCTCCTG |
| pGL3-Control-ING5-MUT-F |
TGAGAGGAATTTTAAGAAGCCCCAAGTGTAAG |
| pGL3-Control-ING5-MUT-R |
CTTACACTTGGGGCTTCTTAAAATTCCTCTCA |
| ING5 over-expression primer |
|
| pcDNA3.1-ING5-F |
CGGGGTACCGCCACCATGGCGACCGCCATGTAC TTGG |
| pcDNA3.1-ING5-R |
CCGCTCGAGCTACTTCTTCTTCCTCTTTTCC |
