Skip to main content
. 2015 Oct 16;6(35):38107–38126. doi: 10.18632/oncotarget.5646

Figure 6. ZNF32 knockdown enhances the toxicity of the pro-oxidant drug PL in vivo.

Figure 6

A. Mice were injected with HepG2 cells stably expressing shZNF32 or shNC. When the diameter of tumor xenografts reached approximately 3 mm, the mice were injected daily with PL (2.5 mg/kg) or DMSO. Mice were euthanized 15 days after treatment. Representative images of the tumors are shown. B. In the box plots, the final tumor weights are presented (n = 8 per group). The whiskers in the box plots represent the maximum and minimum values. C. Tumor volume analysis of xenografts of the indicated cells (n = 8 per group). D. Tumor xenograft tissues were fixed with 4% paraformaldehyde, processed, embedded in paraffin wax and then assessed for cellular apoptosis using a TUNEL assay. E. Oxidative DNA damage in tumor xenograft tissues was detected by quantifying 8-OHdG expression. F. Tumor xenograft tissues were homogenized and then assessed for the lipid peroxidation levels using an MDA assay. The data are presented as the mean values ± SEM. Each experiment was performed at least in triplicate, producing consistent results. *p < 0.05, **p < 0.01, #p < 0.001.