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. 2015 Oct 13;6(35):38210–38224. doi: 10.18632/oncotarget.5690

Figure 4. E2F2 knockdown in human breast cancer increases migration and lung colonization.

Figure 4

E2F2 knockdown in human breast cancer was achieved by transfection of MDA-MB-231 cells with shE2F2. Efficacy of E2F2 knockdown was assayed by western blotting A. with untransfected MDA-MB-231 (Con.), MDA-MB-231 transfected with shScramble (Scr.), shE2F2 construct #3 (C3), shE2F2 construct #4 (C4). Migration of MDA-MB-231 control cells B. and with E2F2 knockdown C. in transwell migration assays revealed that the percentage of cells that migrated across the membrane increased when the level of E2F2 was decreased (D. p < 0.0001). In colonization assays with and without the knockdown, lesions were found in the lungs of mice injected with MDA-MB-231 E. and greatly increased with transfection of shE2F2 F. Quantification of the numbers of metastatic lesions revealed an increased number of metastatic lesions in mice injected with MDA-MB-231 transfected with shE2F2 (G. p = 0.0184).