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. 2015 Oct 13;6(35):38210–38224. doi: 10.18632/oncotarget.5690

Figure 5. PTPRD knockdown in human breast cancer decreases migration and lung colonization.

Figure 5

PTPRD knockdown in human breast cancer was achieved by transfection of MDA-MB-231 cells with shPTPRD. Efficacy of PTPRD knockdown was assayed by qRT-PCR (A. p = 0.01). Migration of MDA-MB-231 control cells B. and with PTPRD knockdown C. in transwell migration assays revealed that the percentage of cells that migrated across the membrane decreased when the level of PTPRD was decreased (D. p < 0.0001). In colonization assays with and without the knockdown, lesions were found in the lungs of mice injected with MDA-MB-231 E. and decreased with injection of MDA-MB-231 transfected with shPTPRD F. Quantification of the numbers of metastatic lesions revealed a decreased number of lesion in mice injected with MDA-MB-231 transfected with shPTPRD (G. p = 0.0009).