Skip to main content
. 2015 Oct 21;6(35):38225–38238. doi: 10.18632/oncotarget.5425

Figure 4. BTZ-resistant cells respond to BCR-related signaling.

Figure 4

A. Jeko1, Mino, and BTZ-resistant cells were incubated with 10 nM BTZ for 12 hr. Phosphorylation levels of PI3K, Akt, mTOR, and p70S6K were assessed by Western blotting. β-actin was used to as a loading control. Jeko1/BTZ and Mino/BTZ cells were transfected for 24 hr using control siRNA, or B. CD79A siRNA, or C. Lyn siRNA followed by 10 nM BTZ for an additional 24 hr. Whole cell lysates were used to detect p-PI3K, p-Akt, p-mTOR, PI3K, Akt, and mTOR expression using Western blotting. β-actin was used as a protein loading control.