Fig 2. Competition of [3H] Lys-des-Arg9-BK (1 nM) binding to hB1R-FLAG by a panel of unlabeled peptides. (verification of the affinity of selected peptides used in fusion protein design for the B1R ligands).
Specific binding values (percent of control) are the means ± S.E.M. of the number of duplicate determinations indicated between parentheses. Derived numerical affinity estimates are reported in Results.