Fig 3. Immunoblots of the cell lysates of HEK 293a cells transiently expressing either EGFP-N₃ (authentic GFP, 27 kDa, 10 μl lysate) or fusion proteins based on EGFP (1 μl lysate per track migrated in a 15% gel).

For each of the fusion protein, the major band is the heaviest and is consistent with the predicted size of the insert (S1-P1: 2.02 kDa; S2-P1: 2.15; S2-P2: 2.12; S3-P1: 1.89; S3-P2: 1.85; S4-P1: 3.79). Representative blot from 3 separate experiments.